Ziziphus spina-christi Leaf-Derived Carbon Dots as a Fluorescence Nanosensor to Evaluate Rifaximin Antibacterial via Inner Filter Effect: Greenness and Whiteness Studies

Rifaximin (RFX) is a non-absorbable antibiotic with broad-spectrum efficacy. It treats travelers’ diarrhea, irritable bowel syndrome, non-systematic bacterial infections, overgrowth and enteric infections. In this work, carbon dots prepared from Ziziphus spina-christi leaves’ powders are utilized as green fluorometric biosensor for the assessment of RFX. The morphological lineaments were recognized by using TEM SEM techniques. manifest fluorescence emission peak at 432 nm after an excitation 366 nm. absorbance band RFX (absorbance peaks 370 443 nm) could be thoroughly overlapped excitation/emission bands produced dots. A tool has been designed validated evaluation reliant on inner filter effect methodology, in which act fluorophore absorber. quenching degree activity depended concentration analytical parameters checked directed successfully applied different pharmaceutical formulations. proposed tool’s greenness eco-friendliness profile was evaluated most recent tool, complementary procedure index (Complex-GAPI) Analytical GREEnness metric (AGREE). Additionally, recently released White Chemistry (WAC) whiteness characteristic—which indicated method’s sustainability—was investigated.